HIV test kit INTRODUCTION The screening test is based on the principle of sandwich immunoassay for a qualitative detection of antibody to human immunodeficiency virus in human serum. SPECIMEN COLLECTION Separate serum and use the serum for testing. If the specimen cannot be tested in time, store the specimen 7 days between 2 to 8°C.Over 7 days, store in a refrigerator or freezer. TEST PROCEDURE Follow the illustration and allow test kit to equilibrate to room temperature prior to testing. 1. Remove the test card from the foil pouch and lay it flat on a clean, dry and non-absorbent surface. 2. Drop serum/plasma specimen into the sample well, wait for 15-30 minutes and read results. Do not read after 30 minutes. Reading Results Positive: In addition to a pink colored control band, a distinct pink colored band will also appear in the test region. Negative: Only one colored band appear on the control region. No apparent band on the test region. Invalid: A total absence of color band in both (T) and (C) regions or no colored band appears on the control (C) region is an indication of procedure error and/or that the test reagent has deteriorated. Repeat with a new test kit.