HBsAb Test Kit Introduction HbsAg is a protein antigen produced by HBV, it cannot be passed to others. But this antigen is the earliest indicator of hepatitis B and frequently identifies infected people before symptoms appear. The test is based on the double antibody sandwich principle for a rapid, qualitative detection of HbsAg in serum. The result is very specific and easy to read. Procedure 1. Allow specimen and test strip to reach room temperature prior to testing. 2. Remove the test strip from the sealed foil pouch. Dip the strip with arrow pointing down into the serum for 15 seconds. Do not immerse the test strip beyond the “Mark Line”. 3. Take the test strip out and lay it on a non-absorbent flat surface. Wait for 30 minutes to read results. Do not attempt to interpret test results after 35 minutes. Reading Results Positive: a distinct colored band will also appear in the testregion.Negative: Only one colored band appear on the control region. No apparent band on the test region.Invalid: A total absence of colored line in both (T) and (C) regions or no colored line appears on the control (C) region. The test failed, or the test procedure was not followed properly. Verify the test procedure and repeat the test with a new testing device.